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KMID : 0545120170270122151
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 12 p.2151 ~ p.2155
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Isolation and Characterization of a Novel Broad-host-range Bacteriophage Infecting Salmonella enterica subsp. enterica for Biocontrol and Rapid Detection
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Choi In-Young
Lee Ju-Hoon
Kim Hye-Jin
Park Mi-Ju
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Abstract
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Bacteriophages have gained substantial attention as biocontrol and biorecognition agents, substituting antibodies. In this study, a Salmonella Enteritidis-specific bacteriophage, KFS-SE1, was isolated, identified, and characterized. This Siphoviridae phage infects S. Enteritidis with high specificity. This phage is highly stable under various pH (5?11), temperature (4?60°C), and organic solvent conditions. The KFS-SE1 genome consisted of 59,715 bp with 73 predicted open reading frames and 57.14% GC content; it had a complete set of genes required for phage reconstruction. Comparative phylogenetic analysis of KFS-SE1 revealed that it was very similar to the other Salmonella phages in the Siphoviridae family. These characteristics suggest that KFS-SE1 with its high specificity and host lysis activity toward S. Enteritidis may have various potential applications.
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KEYWORD
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Bacteriophage, Salmonella Enteritidis, biocontrol, rapid detection
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